Versão em português
N.º 23
2016
pp. 71-77

 

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Article
Development of new approaches for the detection of yeast and bacteria thriving in mortars

Ricardo Vieira1,*, Marina González-Pérez1, António Pereira1,2, António Candeias1,2, Ana T. Caldeira1,2

1 Laboratório HERCULES, Universidade de Évora, Évora, Portugal

2 Departamento de Química, Escola de Ciência e Tecnologia, Universidade de Évora, Évora, Portugal

* ricardo.o.vieira89@gmail.com

Abstract

The development of simple and rapid new approaches for analysing microbial communities colonising Cultural Heritage materials is pivotal for its safeguard. Fluorescence in situ hybridisation technique using ribosomal RNA directed probes (RNA-FISH) has demonstrated a great potential for this purpose. A protocol for analysing filamentous fungi in mortars has been already developed in previous studies. In this work this protocol has been adapted for detecting bacteria and yeasts. Good results have been obtained for the analysis of suspensions of isolates. In this way, the optimized protocol was applied in microsamples from synthetic mortar artificially inoculated with yeast and bacterial isolates. Promising results have been obtained for the ex situ analysis of yeast and bacteria thriving in mortar microsamples.

Keywords

Fluorescence in situ hybridisation
Cultural Heritage
Microbial communities

Language

Portuguese

Original title

Novos desenvolvimentos para a deteção de leveduras e bactérias presentes em argamassas

DOI

10.14568/cp2015047

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Citation

Conservar Património | APA | Chicago | NP405 | ABNT

Conservar Património:
Vieira, R.; González-Pérez, M.; Pereira, A.; Candeias, A.; Caldeira, A. T., 'Novos desenvolvimentos para a deteção de leveduras e bactérias presentes em argamassas', Conservar Património 23 (2016) 71-77, https://doi.org/10.14568/cp2015047.

APA:
Vieira, R., González-Pérez, M., Pereira, A., Candeias, A., & Caldeira, A. T. (2016). Novos desenvolvimentos para a deteção de leveduras e bactérias presentes em argamassas. Conservar Património, 23 71-77. DOI:10.14568/cp2015047.

Chicago:
Vieira, Ricardo, Marina González-Pérez, António Pereira, António Candeias, and Ana T. Caldeira. 2016. "Novos desenvolvimentos para a deteção de leveduras e bactérias presentes em argamassas." Conservar Património 23:71-77. doi:10.14568/cp2015047.

NP405:
VIEIRA, Ricardo [et al.] – Novos desenvolvimentos para a deteção de leveduras e bactérias presentes em argamassas. Conservar Património. [Em linha]. 23 (2016) 71-77 [Consult. ]. Disponível em WWW: <URL:https://doi.org/10.14568/cp2015047>. ISSN 21829942.

ABNT:
VIEIRA, Ricardo et al. Novos desenvolvimentos para a deteção de leveduras e bactérias presentes em argamassas. Conservar Património, Lisboa, v. 23, p. 71-77, 2016. Disponível em: <https://doi.org/10.14568/cp2015047>. Acesso em: .

History

Received: 2015-12-28
Revised: 2016-5-25
Accepted: 2016-7-14
Online: 2016-7-27
Publication: 2016-7-28

References

1 Sterflinger, K.; Piñar G., 'Microbial deterioration of cultural heritage and works of art-tilting at windmills?', Applied Microbiology and Biotechnology 97(22) (2013) 9637-9646, doi:10.1007/s00253-013-5283-1.

2 Dakal, T.; Arora, P., 'Evaluation of potential of molecular and physical techniques in studying biodeterioration', Reviews in Environmental Science and Bio/Technology 11(1) (2012) 71-104, doi:10.1007/s11157-012-9264-0.

3 Otlewska, A.; Adamiak, J.; Gutarowska, B., 'Application of molecular techniques for the assessment of microorganism diversity on cultural heritage objects', Acta Biochimica Polonica 61(2) (2014) 217-225.

4 Bottari, B.; Ercolini, D.; Gatti M.; Neviani, E., 'Application of FISH technology for microbiological analysis: current state and prospects', Applied Microbiology and Biotechnology 73(3) (2006) 485-494 doi:10.1007/s00253-006-0615-z.

5 Moter, A.; Göbel, U. B., 'Fluorescence in situ hybridization (FISH) for direct visualization of microorganisms', Journal of Microbiological Methods 41(2) (2000) 85-112, doi:10.1016/S0167-7012(00)00152-4.

6 Amann, R.; Fuchs B. M.; Behrens, S., 'The identification of microorganisms by fluorescence in situ hybridisation', Current Opinion in Biotechnology 12(3) (2001) 231-236, doi:10.1016/S0958-1669(00)00204-4.

7 Souza, J.; Moreira da Silva, R.; Koshikene, D.; Silva, E., 'Applications of fluorescent in situ hybridization (FISH) in environmental microbiology', Journal of Food Agriculture and Environment 5 (2007) 408-411.

8 Xufre, A.; Albergaria, H.; Inácio, J.; Spencer-Martins, I.; Gírio, F., 'Application of fluorescence in situ hybridisation (FISH) to the analysis of yeast population dynamics in winery and laboratory grape must fermentations', International Journal of Food Microbiology 108(3) (2006) 376-384, doi:10.1016/j.ijfoodmicro.2006.01.025.

9 Douterelo, I.; Boxall, J. B.; Deines, P.; Sekar, R.; Fish, K. E.; Biggs, C. A., 'Methodological approaches for studying the microbial ecology of drinking water distribution systems', Water Research 65(0) (2014) 134-156, doi:10.1016/j.watres.2014.07.008.

10 Kempf, V. A. J.; Trebesius, K.; Autenrieth, I. B., 'Fluorescent in situ hybridization allows rapid identification of microorganisms in blood cultures', Journal of Clinical Microbiology, 38(2) (2000) 830-838, http://jcm.asm.org/content/38/2/830 (acesso em 2016-07-25).

11 Urzì, C.; La Cono, V.; De Leo, F.; Donato, P., 'Fluorescent in situ hybridization (FISH) to study biodeterioration', in International Congress on Molecular Biology and Cultural Heritage, Balkema Publishing, Sevilha (2003) 55-60.

12 Villa, F.; Cappitelli, F.; Principi, P.; Polo, A.; Sorlini, C., 'Permeabilization method for in-situ investigation of fungal conidia on surfaces', Letters in Applied Microbiology 48(2) (2009) 234-240, doi:10.1111/j.1472-765X.2008.02520.x.

13 Piñar, G.; Ramos, C.; Rölleke, S.; Schabereiter-Gurtner, C.; Vybiral, D.; Lubitz, W.; Denner, E. B. M., 'Detection of indigenous halobacillus populations in damaged ancient wall paintings and building materials: molecular monitoring and cultivation', Applied and Environmental Microbiology 67(10) (2001) 4891-4895, doi:10.1128/aem.67.10.4891-4895.2001.

14 Baskar, S.; Baskar, R.; Mauclaire, L.; McKenzie, J. A., 'Microbially induced calcite precipitation in culture experiments: Possible origin for stalactites in Sahastradhara caves, Dehradun, India', Current Science 90(1) (2006) 58-64, http://www.iisc.ernet.in/currsci/jan102006/58.pdf (acesso em 2016-07-25).

15 Santos, A.; Cerrada, A.; García, S.; San Andrés, M.; Abrusci, C.; Marquina, D., 'Application of molecular techniques to the elucidation of the microbial community structure of antique paintings', Microbial Ecology 58(4) (2009) 692-702, doi:10.1007/s00248-009-9564-2.

16 Vieira, R.; Nunes, P.; Martins, S.; González, M.; Rosado, T.; Pereira, A; Candeias, A.; Caldeira, A., 'Fluorescence in situ hybridisation for microbiological detection in mortars', in Science, Technology and Cultural Heritage, ed. A. Rogerio-Candelera, Taylor & Francis, London (2014) 257-262.

17 Kalyuzhnaya, M.; Zabinsky, R.; Bowerman, S.; Baker, D.; Lidstrom, M.; Chistoserdova, L., 'Fluorescence in situ hybridization-flow cytometry-cell sorting-based method for separation and enrichment of type i and type ii methanotroph populations', Applied and Environmental Microbiology 72(6) (2006) 4293-4301, doi:10.1128/AEM.00161-06.

18 Fuchs, B. M.; Wallner, G.; Beisker, W.; Schwippl, I.; Ludwig, W.; Amann, R., 'Flow cytometric analysis of the in situ accessibility of Escherichia coli 16S rRNA for fluorescently labeled oligonucleotide probes', Applied and Environmental Microbiology 64(12) (1998), 4973-4982, http://aem.asm.org/content/64/12/4973 (acesso em 2016-07-25).

19 Fuchs, B. M., Syutsubo, K.; Ludwig, W.; Amann, R., 'In situ accessibility of Escherichia coli 23S rRNA to fluorescently labeled oligonucleotide probes', Applied and Environmental AEM.67.2.961-968.2001.

20 Bottari, B.; Ercolini, D.; Gatti M.; Neviani E., 'Application of FISH technology for microbiological analysis: current state and prospects', Applied Microbiology and Biotechnology 73(3) (2006) 485-494 doi:10.1007/s00253-006-0615-z.

Stable url: https://doi.org/10.14568/cp2015047


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