Versão em português
N.º 17
2013
pp. 11-20

 

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Article
Microbiological diversity in the Unfinished Sacristy building of the Convent of Christ, Tomar, and evaluation of its biocide-based control

Dina M. R. Mateus1,2,*, Renata B. Silva1, Fernando M. C. Costa1, João P. F. Coroado1,2

1 Instituto Politécnico de Tomar, Campus da Quinta do Contador, Estrada da Serra, 2300-313 Tomar, Portugal

2 GeoBiotec - Geobiociências, Geotecnologias e Geo-engenharias, Universidade de Aveiro

* dinamateus@ipt.pt

Abstract

This laboratory research aimed at characterizing the microorganisms that colonize the limestone walls of the Unfinished Sacristy of the Convent of Christ (Tomar, Portugal), as well as to study their control by means of biocidal agents. Six different locations were sampled through swabbing and the microorganisms were characterized using classical microbiology techniques. The presence of fungi, heterotrophic bacteria and autotrophic microorganisms was verified in all samples. The relative effectiveness of different biocides was tested through the analysis of the inhibition halos they caused in the growth plates of bacterial and fungal isolates. For bacteria, two currently used biocides were tested: Biotin T and benzalkonium chloride. For fungi, these same two biocides plus a paint additive were assessed. The biocides showed different levels of effectiveness for different microorganisms: the benzalkonium chloride was more effective on bacterial isolates, while Biotin T showed a better performance on fungal isolates.

Keywords

Stone decay
Biodeterioration
Bacteria
Fungi
Biocides

Language

Portuguese

Original title

Diversidade microbiológica do edifício da Sacristia Incompleta do Convento de Cristo, em Tomar, e avaliação do seu controlo por biocidas

DOI

10.14568/cp2012005

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Citation

Conservar Património | APA | Chicago | NP405 | ABNT

Conservar Património:
Mateus, D. M. R.; Silva, R. B.; Costa, F. M. C.; Coroado, J. P. F., 'Diversidade microbiológica do edifício da Sacristia Incompleta do Convento de Cristo, em Tomar, e avaliação do seu controlo por biocidas', Conservar Património 17 (2013) 11-20, https://doi.org/10.14568/cp2012005.

APA:
Mateus, D. M. R., Silva, R. B., Costa, F. M. C., & Coroado, J. P. F. (2013). Diversidade microbiológica do edifício da Sacristia Incompleta do Convento de Cristo, em Tomar, e avaliação do seu controlo por biocidas. Conservar Património, 17 11-20. DOI:10.14568/cp2012005.

Chicago:
Mateus, Dina M. R., Renata B. Silva, Fernando M. C. Costa, and João P. F. Coroado. 2013. "Diversidade microbiológica do edifício da Sacristia Incompleta do Convento de Cristo, em Tomar, e avaliação do seu controlo por biocidas." Conservar Património 17:11-20. doi:10.14568/cp2012005.

NP405:
MATEUS, Dina M. R. [et al.] – Diversidade microbiológica do edifício da Sacristia Incompleta do Convento de Cristo, em Tomar, e avaliação do seu controlo por biocidas. Conservar Património. [Em linha]. 17 (2013) 11-20 [Consult. ]. Disponível em WWW: <URL:https://doi.org/10.14568/cp2012005>. ISSN 21829942.

ABNT:
MATEUS, Dina M. R. et al. Diversidade microbiológica do edifício da Sacristia Incompleta do Convento de Cristo, em Tomar, e avaliação do seu controlo por biocidas. Conservar Património, Lisboa, v. 17, p. 11-20, 2013. Disponível em: <https://doi.org/10.14568/cp2012005>. Acesso em: .

History

Received: 2012-10-22
Revised: 2013-02-13
Accepted: 2013-03-08
Online: 2013-07-22
Publication: 2013-7-22

References

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21 Pinheiro, A. C.; Macedo, M. F.; Juradoc, V.; Saiz-Jimenez, C.; Viegas C.; Brandão J.; Rosado L., 'Mould and yeast identification in archival settings: Preliminary results on the use of traditional methods and molecular biology options in Portuguese archives', International Biodeterioration and Biodegradation 65 (2011) 619-627.

Stable url: https://doi.org/10.14568/cp2012005


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